Pipette, also known as the liquid transfer gun, is a measuring tool to move the liquid from the original container to another container within a certain range. It is widely used in biology, chemistry and other fields.
Precautions for using pipettes
(1) When sucking liquid, be sure to release your thumb slowly and steadily, and never let it go suddenly, to prevent the solution from being sucked in too fast and rushing into the liquid dispenser to corrode the plunger and cause air leakage.
(2) In order to obtain higher accuracy, the tip needs to absorb the sample solution once before formally pipetting, because when absorbing serum protein solution or organic solvent, a "liquid film" will remain on the inner wall of the tip, causing liquid drainage. The amount is too small and causes errors.
(3) Liquids with high concentration and viscosity will produce errors. The compensation amount to eliminate the error can be determined by experiments. The compensation amount can be set by changing the reading in the reading window with the adjustment knob.
(4) The pipette can be calibrated by weighing the weight of the pure water taken with an analytical balance and calculating. 1mL of distilled water weighs 0.9982g at 20°C.
(5) It is very undesirable to tighten the pipette by repeatedly hitting the tip. Long-term operation will cause the internal parts to loosen and damage the pipette.
(6) Do not pipette when the pipette is not equipped with a suction tip.
(7) Please pay attention when setting the measuring range? Rotate to desired range? Are the numbers clearly in the display window? If the set range is within the range of the pipette gun, do not turn the button out of the range, otherwise the mechanical device will be jammed and the pipette gun will be damaged.
(8) The pipette is strictly prohibited from absorbing highly volatile and corrosive liquids (such as concentrated acid, concentrated alkali, organic matter, etc.).
(9) It is strictly prohibited to use a pipette to mix the liquid.
(10) Do not use a large-capacity pipette to transfer small volumes of liquid to avoid affecting accuracy. At the same time, if you need to transfer a larger amount of liquid outside the measuring range, please use a pipette.
Daily maintenance of pipettes
1.If it is not used for a long time, the measuring range of the pipette should be adjusted to the maximum scale to keep the spring in a relaxed state to protect the spring;
2.Clean the outer wall of the pipette regularly, you can use 95% alcohol or 60% isopropyl alcohol, then wipe it with distilled water and let it dry naturally;
3.Check whether there is any leakage when using. The method is to absorb the liquid and place it vertically for a few seconds to see if the liquid level drops. If there is leakage, check whether the suction nozzle matches and whether the spring piston is normal;
4.When there is liquid in the pipette nozzle, it is strictly prohibited to place the pipette horizontally or upside down to prevent liquid from flowing into the piston chamber and corroding the pipette piston;
5.Use the correct method to install the suction head. Remember not to use excessive force, let alone chop the suction head for installation.
