Luminescence (Lumi)
The photons generated by the luminescence reaction are converted into electrical signals for detection by PMT in the reader. The signal collected by instrument covers the entire spectrum and allows to do glow-type luminescence. Luminescent detection provides high sensitivity and wide dynamic range to the assay,which does not require a light source or specific optics for excitation, thus it is optically simpler than fluorescence detection.
Light-induced chemiluminescence (LiCA)
LiCA technology is a homogeneous affinity detection method based on a pair of microbeads that can be used for almost all research applications. LiCA no-wash assays provide simple, rapid,and highly sensitive detection of biomolecules in cell lysates,cell supernatants, serum,and various other types of samples,as well as binding assays with a broad affinity range.LiCA detection technology can complete 96-well plate detection in minutes,while maintaining a very high signal-to-background ratio,enabling fast, highly sensitive detection techniques.
Time-resolved fluorescence(TRF)
TRF provides higher signal-to-noise ratios,excellent sensitivity&stability,wider dynamic range,as well as flexibility,even in limited or very low sample volumes.TRF detection uses the fluorescence retention effect of lanthanide chelates to reduce the interference of background fluorescence in the reaction that significantly improve the dynamic range and has high signal stability.
● Using dual-mode photon counting acquisition and correction technology,through the patented photon counting linear fitting technology,the commonly used photon counter with a dynamic range of 10^5 can be expanded to 10^7,expanding the dynamic/linear range by 100-fold and adapting to a wider range of concentration gradient experiments.
● The con-focal optical system can effectively suppress the interference of ambient stray light,and minimize the cross-interference caused by the difference in high and low concentrations between wells.
● In the TRF detection mode,the combination of dual excitation optical paths and high dynamic range PMT technology can ensure that weak signals are detected, while strong signals do not enter the saturation state, and the maximum dynamic range and linear range can be obtained at one timeIn the LiCA mode,laser is used for excitation, which can minimize the influence of non-specific fluorescence in the microplate or reaction sample excited by other light.
● With a built-in incubator for temperature control up to 45°C, I96 provides a uniform incubation and prevents condensation. It is well-suited for temperature-critical applications,including certain enzyme assays and cell-based applications.
● I96 comes with an analysis software,its easy-to navigate interface will guide you through the measurement process and help you get the results you need.A variety of application schemes and experimental templates are preset in the software, suitable for most common applications.Support different measurement modes such as standard curve quantification, end-point method, kinetic analysis, etc.
● I96 has power-on self-test and auto-calibration functions for reliable results.
Automatic dynamic range, which selects optimal reading range based on the signal intensity
The instrument supports 8*12 opaque microplate and automation-friendly plate carrier guarantee an easy integration into robotic platform.
Small footprint meets the requirements of small space configuration, saving the laboratory space as much as possible.