High-performance liquid chromatography (HPLC) is a reliable and established technique used in laboratories to separate, identify, and quantify components in a mixture, HPLC techniques can be applied to samples such as environmental, forensic, pharmaceuticals, food, and industrial chemicals.
As a universal solvent, water is used in mobile phases and sample preparation in HPLC analysis, but ordinary tap water cannot be used for HPLC analysis due to the presence of contamination, that is why ultra-pure water, also called HPLC water or ASTM Type 1 water by Innova Nova U and Performa U water purification system is needed in HPLC analysis.
What Is the Standard of HPLC Grade Ultra-pure Water?
HPLC-grade water is ultra-pure water with low UV absorbance. Japanese Standard Association Group request HPLC analysis water should be purified by a combination of reverse osmosis, ion exchange, distillation, filtration, ultraviolet irradiation, and other methods. also, The American Society for Testing and Materials (ASTM) describes the standard specifications here:
Resistivity: >18mΩ at 250℃
TOC: <10ppb,
Bacteria: <1CFU/ml
HPLC water is needed at the time of preparation of aqueous mobile phases, buffers, and solutions of samples and standards, and laboratories can source HPLC-grade water from Innova Nova U and Performa U water purification Systems.
How Innova Water Purification System Offer Support for HPLC Grade Ultra-pure Water?
Innova water purification Systems is an ideal equipment for HPLC analysis water supply. The Type I ultra-pure water produced by Innova Nova U and Performa U water purification system meets or even exceeds the standard of HPLC water.
1.Lower the ion contamination and makes the resistivity reach 18.2MΩ.cm
Corrosive ions would affect the service life of high-pressure infusion pumps and other accessories. Nuclear-grade ion exchange resin in Innova pre-purification cartridge and ultra purification cartridge can control the ions in water at a trace level, then the ion contamination become lower and makes the resistivity reach 18.2MΩ.cm.
2.Solve the particulate pollution problem
Particles could damage the booster pump or block the membrane, even more, it may also change the composition of the sample. With the help of Innova pre-purification cartridge A, Innova could remove all particles bigger than 0.22μm, which could avoid all lower particulate matter pollution.
3.TOC < 2ppb(μg/L)
Lower TOC help to keep the chromatographic baseline stable and ensure the service life of string media. Innova ultra-pure water system is equipped with 185nm & 254nm dual-wavelength UV lamps, the 185nm UV lamp can oxidize trace organic matter in water into carbon dioxide and water, and can kill bacteria. Innova Nova U water purification systems equipped with a built-in on-line TOC analyzer,total organic carbon (TOC) data will be shown on the display screen and evaluated by the TOC analyzer in real time, TOC level of type I water is always less than 2ppb(µg/L).
4.Low Bacteria contamination <0.01cfu/mL
Bacteria would cause organic molecules contamination and column blockage.Under the joint action of Innova pre-purification cartridge and 254nm UV lamp，bacteria contamination would be under 0.01cfu/mL,thus the flow rate and the baseline is stable,avoid the chromatographic noise peak and HPLC analysis be interferenced.
Innova offers a large variety of lab equipment and has been committed to delivering the highest quality lab equipment to medical device companies and laboratories worldwide. To learn more, visit our website https://www.innovabiomed.com, or contact us via info@innobiomed.com.
