What is Molecular Diagnosis?
Molecular diagnostics is based on the study and analysis of human genes so that we can better understand diseases, It is also called molecular pathology, and involves taking DNA or RNA, the unique genetic code found in our cells, and analyzing the sequences for red flags that can pinpoint the potential emergence of a specific disease.With the help of numerous scientific research equipment, This exciting field of genetics is leading us towards a more personalized medicine with a proactive approach.
What Equipment are Needed in A Molecular Diagnostic Lab?
There are specific equipment needed in a molecular diagnostic lab in order to analyze possible red flags in the sequencing of genetic codes. A molecular diagnostic lab typically includes the following supplies and equipment:
1. Thermal Cycler
There are different types of PCR machines, which are used for different experiments. Ordinary and gradient PCR machines are used for DNA/RNA amplification in the process of gene cloning and gene detection. Fluorescence quantitative PCR instrument is used for nucleic acid quantitative analysis, gene expression differential analysis, single nucleotide polymorphism detection, and methylation detection.
2. Refrigerator
A refrigerator is an essential instrument for storing reagents and samples in the laboratory. Some reagents used in molecular biology experiments are required to be stored at 4 degrees, and some are required to be stored at minus 20 degrees. The experimenter must clearly see the storage conditions of the reagents and store them at an appropriate temperature. Specifically, items stored at different temperatures are as follows:
a. 4°C is suitable for storing certain solutions, reagents, medicines, etc.
b. -20°C is suitable for some reagents, medicines, enzymes, serum, prepared antibiotics and DNA, protein samples, etc.
c.-80°C is suitable for some long-term cryopreserved samples, Escherichia coli strains, purified samples, special low-temperature-treated digestive juices, and competent storage.
d. Chromatography freezer at 0-10°C is suitable for electrophoresis, chromatography, dialysis and other experiments under low temperature conditions.
3. Biosafety Cabinet
Many reagents and samples involved in molecular experiments are poisonous, which is more harmful to operators. In order to prevent the spread of harmful suspended particles and aerosols, biological safety cabinets can be used to provide safety protection for operators, samples and cross-infection between samples and the environment.
4. Water Purification System
The Water purification system includes distilled water and pure water machine. The price of the water distiller is cheap, but someone needs to be on duty during the water making process; the price of the pure water machine is higher, but it is easy to use and can store a certain amount of pure water. There are also different levels of pure water used. Generally, pure water is required for experimental water, and ultra pure water is required for PCR, DNA sequencing, and enzyme reactions.
5. Pipette
A pipette is a laboratory tool commonly used in chemistry, biology, and medicine to transport a measured volume of liquid, often as a media dispenser. Pipettes come in several designs for various purposes with differing levels of accuracy and precision, from single-piece glass pipettes to more complex adjustable or electronic pipettes.
6. Autoclave
Autoclaves are used in molecular diagnostic labs to sterilize waste and lab equipment. Sterilization is accomplished by using heat to kill bacteria and spores.
How will Innova Real-Time PCR Provides Support for Molecular Diagnosis?
Real-time fluorescence quantitative PCR detection system, as a necessary choice for quantitative analysis of molecular biology, has been widely used in hospitals, universities, research institutions, and quarantine departments for molecular diagnosis and research. Real-Time PCR is also widely used in the diagnosis of infectious diseases, genetic diseases, cancers characterized by the detection of nucleic acid DNA/RNA, as well as the improvement of genetically modified organisms, animal and plant quarantine, animal sex determination, etc. Real-Time PCR is playing its unique role in quantitative diagnosis, ranging from viral colds to severe infectious diseases.
INNOVA Real-Time PCR System has strong specificity, high sensitivity, good stability, and is simple, fast, easy to operate.
• Up to 6 fluorescence detection channels allowing multiplex PCR
• Effectively reduce multi-color crosstalk and edge effect, no ROX correction required to reduce sample and reagent use
• Innovative scanning method and time-resolved signal separation technology to improve detection sensitivity
• Unique edge temperature compensation technology to minimize "edge effect"
• User-friendly software
• Innovative technology with long-lasting LED light provides reliability results
Innova offers a large variety of molecular diagnostic lab equipments, and has been committed to delivering the highest quality lab equipment to medical device companies and laboratories worldwide. To learn more, visit our website https://www.innovabiomed.com, or contact us via info@innobiomed.com.
