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Centrifugation knowledge

Centrifugation knowledge

November 16, 2023

Centrifuges generate centrifugal force by rotating a rotor driven by a motor. It uses centrifugal force to separate components in a mixture of liquid and solid particles or liquid and liquid.Mainly used to separate solid particles from liquids in suspension or to separate two liquids with different densities and immiscible in emulsion.


The denser particles move in the direction away from the axis and the less dense particles move towards the axis. These denser particles are deposited at the bottom of the centrifuge tube, forming a precipitate, which results in solid-liquid separation or liquid-liquid separation.

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  • Centrifuge' s main parameters:

Speed: Centrifugal speed is often measured in rpm-revolutions per minute(r/min), which is the number of revolutions per minute.

Centrifugal force(F): Centrifugal force is related to the radius of the centrifuge' s rotor, as different centrifuges have different rotor sizes, so that at the same rpm speed, the force generated will be different.



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RCF(Relative centrifugal force):The actual centrifugal force is converted to a multiple of the acceleration due to gravity and is expressed as a number multiplied by "g", e.g. 3000xg. 

-Two expression approaches:

1.Positive or negative deviation of rotational speed,

e.g. ±20rpm

2.Percentage (1%) deviation of max speed,

e.g. 4000rpm x 1% = ±40rpm

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  • Levelling principles for centrifuges:

Weight balancing is very important in centrifugation.When placing the centrifuge tubes into the rotor, it is important to make sure that the weight of each sample is the same as the sample in another relative position.

It is similar to balancing a balance.The rotor of a centrifuge has a high kinetic energy in its high-speed motion, and if it is not balanced properly, the rotor can get out of the position it is in, causing danger;




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