With the combination of biochip technology and fluorescence probe quantification technology,
the application prospects of real-time fluorescence quantitative PCR in medical detection and various other fields are very broad.
Technical Introduction
RT-QPCR refers to the addition of fluorescent dye SYBR or specific probes labeled with fluorescence in the PCR reaction system, and the real-time online monitoring of the PCR reaction process using the accumulation of fluorescence signals.
Due to the linear relationship between the Ct value of the PCR amplified gene and the initial copy number of the template, it becomes a quantitative basis and can achieve relative or absolute quantification of the target gene in the sample.
Fluorescent quantitative PCR not only achieves absolute quantification, but also has characteristics such as sensitivity and specificity, high energy for multiple reactions, high automation, pollution-free, real-time, and accuracy.
Method introduction
According to Real time qPCR, there are two types of fluorescent chemicals used: fluorescent dyes and fluorescent probes.
Depending on the different fluorescent substances used,
the commonly used methods for this technology include SYBR Green dye method and TaqMan probe method
Operation method
· Design specific primers or fluorescent probes based on the target gene sequence;
· Extract DNA/RNA, RNA reverse transcription, electrophoresis, and concentration measurement from each sample;
· Degenomic DNA, RNA reverse transcription;
· Configure a PCR reaction system and add the necessary solutions for templates, primers, dyes, and dNTP systems;
· RealTime PCR amplification；
· Obtain offline data and analyze amplification curves, dissolution curves, and expression differences.
INNOVA Real-time Quantitative PCR
IRTP96 is 96-wells Real-Time Quantitative PCR system. Based on innovative signal gathering and spectrum separation technology,
together with unique temperature control technology,
it reached an advanced level in terms of detection sensitivity, multi-color crosstalk correction, temperature uniformity and accuracy.
The instrument is available with a 4-channel or a 5-channel configuration and is compatible with a wide spectrum of qPCR dye-based and probe-based chemistry,
easily realizing multiplex PCR applications.
The instrument supports standard analysis methods like absolute quantification, genotype, HRM, MMCA, and gene expressing analysis.
Product Features
● Simultaneously testing 96 samples
● The instrument is equipped with 4 fluorescence detection channels. If 5 channels are needed, an additional CY5.5 channel can be configured:
● Intuitive software operation interface, no need to learn, simple and easy to use.
● Ultra fast temperature rise and fall rate, up to 10 degrees per second, scanning time of 1 second,
can complete 45 cycles of amplification and detection within 25 minutes, greatly saving PCR detection time and improving detection efficiency.
● Excellent temperature accuracy (± 0.1 °C) and uniformity (± 0.15 °C)
● A sensitive signal detection system ensures multi-channel fluorescence detection without crosstalk
The signal detection system uses a unique Fresnel lens, an efficient PMT detector, and an LED light source.
The time-resolved scanning system detects multicolor crosstalk without the need for ROX correction.
